Fig. 7.

S1PR1 downregulation account for T cell residence in SLOs. a, b Four weeks after parabiosis surgery, blood, cervical LNs (cLNs), mesenteric LNs (mLNs), spleen, and Peyer’s patches (Pp) were recovered and analyzed. a S1PR1/CD69 representative dot-plots are shown for blood and cLN CD4 Treg, Tmem, and Tnaive cells from a representative parabiont. b Proportions of host cells (CD45.1⁺ for the CD45.1 parabiont and CD45.2⁺ for the CD45.2 parabiont) among the indicated CD4 Treg (upper panel) and Tmem (lower panel) cell-subsets recovered from cLNs, mLNs, spleen, and Peyer’s patches (Pp) are shown as means ± SEM with paired t-test for six parabiotic pairs from three independent experiments. c pLNs, mLNs, spleen, and Peyer’s patches (Pp) of 1- and 12-month-old mice were recovered and analyzed. Proportion of the indicated CD4 Treg (upper panel) and CD4 Tmem (lower panel) cell-subpopulations are shown as means with unpaired t-test. *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant