Fig. 5.

Analysis of gene knockout effects in HCT116 cells and gene expression changes in melanoma associated with corin treatment. a Knockout of LSD1 in HCT116 cells decreased the rate of cancer cell proliferation by ~40% (n = 4). b, c Knockout of LSD1 enhanced the potency of MS-275 but did not affect the potency of corin in HCT116 cells as determined by [³H]thymidine incorporation after 48 h treatment (n = 4). d, e Venn diagrams depicting increases in global and tumor suppressor gene transcription (fold change ≥ 2σ, p < 0.05), respectively, upon 2.5 µM MS-275 or corin treatment (n = 3). f, g qRT-PCR validation of gene expression changes identified by microarray. WM983B melanoma cells were treated with inhibitor (2.5 µM) for 24 h prior to RNA isolation and analysis (n = 3). h ChIP-PCR localized CoREST complex components HDAC1 and LSD1 to the promoter region of CHOP (n = 3). i ChIP-PCR localized HDAC1, but not LSD1, to the promoter region of SYN1 (n = 3). Data (mean ± SEM) are representative of at least three independent experiments (unpaired t test, *p < 0.05, ***p < 0.001)