Fig. 3.

SCFA protect from postmenopausal bone loss. a Representative µCT images of the trabecular part of tibial bone of WT sham operated (Sham) or ovariectomized (OVX) mice fed with C2, C3, or C4 in the drinking water for 8 weeks or left untreated (not treated, NT). b Bone volume per total volume (BV/TV) and c trabecular separation (Tb.Sp.) analyzed by µCT. d Immunohistological analysis of TRAP-stained osteoclasts in the tibia of mice showing the number of osteoclasts per analyzed bone parameter (N.Oc./B.pm). e Serum CTX-I bone resorption and f osteocalcin (OCN) bone formation marker levels measured by ELISA. Data are expressed as the mean ± s.d. Pictures are representative of at two independent experiments. Statistical difference was determined by one-way ANOVA. *p < 0.5; **p < 0.01; ***p < 0.001