Fig. 7.

Inactivation of Twinkle in astrocytes leads to microglial activation and demyelination. Immunofluorescent staining of mid-sagittal sections from 6 months old mice, brain graph shows sampling site. a Immunofluorescent staining of microglia (IBA1, red) and nucleus (DAPI, blue) in cortex. b Myelin content (MBP, green), oligodendrocytes (Olig2, red), and nuclei (DAPI, blue). c Pan-axonal staining of long axons in corpus callosum. d Quantification of Olig2-positive cells and length of processes of IBA1-positive cells in cortex; western blot of MBP, and its quantification. Insets: enlarged boxed area. Scale bars: 20 μm (a), 50 μm (b, c). The data are presented as mean and error bars indicate standard deviation. Statistical significance: *p < 0.05, ***p < 0.001