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. 2017 Dec 15;196(12):1571–1581. doi: 10.1164/rccm.201612-2480OC

Figure 4.

Figure 4.

Idiopathic pulmonary fibrosis (IPF) fibroblasts exhibit enhanced glycolysis and increased extracellular mitochondrial DNA. Compared with normal human lung fibroblasts (NHLFs) (left), IPF fibroblasts (right) displayed enhanced aerobic glycolysis, as measured by a significantly elevated (A) extracellular acidification rate (ECAR) and (B) ECAR/oxygen consumption rate (OCR) ratio. Data are presented as mean (±SEM) ECAR (mpH/min) and mean (±SEM) ratio of ECAR/OCR, respectively. (C) Relative to samples obtained from NHLFs (left), a significant increase in MT-ATP6 concentration was detected in supernatants from IPF fibroblasts (right). Data are presented graphically as log base 10 of the raw values (MT-ATP6 copies per microliter of supernatant) with mean (±SEM). A graph including the raw values is presented in Figure E4B. (D) Cell counts were unchanged between NHLFs (left) and IPF fibroblasts (right). Data are presented as mean (±SEM) fold change in cell count. (E) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay demonstrated no significant differences in the viability of NHLFs (left) and IPF fibroblasts (right). Data are presented as mean (±SEM) fold change in absorbance.