Fig. 3.

Association of the NLRP3 rs10754558 polymorphism with IL1β mRNA levels in PBMCs and serum IL1β levels from patients with GA. The levels of IL1β mRNA in PBMCs and IL1β in serum were measured in different genotype carriers from patients with GA. Significant differences were observed among the three genotypes of rs10754558 with respect to IL1β mRNA and serum IL1β levels both from patients with acute and non-acute GA (IL1β mRNA: F = 30.44, 23.61, all P < 0.0001, respectively; IL1β: F = 65.33, 44.64, all P < 0.0001, respectively). a, b In patients with acute GA, the IL1β mRNA and serum IL1β levels in the 45 GG homozygotes and 45 heterozygotes carriers were significantly increased compared with 45 CC homozygotes carriers (all P < 0.01), and IL1β mRNA and serum IL1β levels were higher in GG homozygotes carriers than in CG heterozygotes carriers (all P < 0.01). c In patients with non-acute GA, IL1β mRNA levels were much higher among the 45 GG and 45 CG genotype carriers than in the 45 CC genotype carriers (all P < 0.01); no difference was observed between the GG and CG genotypes (P > 0.05). d In patients with non-acute GA, serum IL1β levels in the 45 GG homozygotes and 45 heterozygotes carriers was significantly increased compared with the 45 CC homozygotes carriers (all P < 0.01), and serum IL1β levels were higher in the GG homozygotes carriers than in the heterozygotes carriers (P < 0.01). The data are shown as box plots. Each box represents the upper and lower interquartile range (IQR). The whiskers represent 1.5 times the upper and lower IQRs. The ANOVA, LSD method was performed. ^a P < 0.01 in comparison with patients with the CC genotype; ^b P < 0.01 in comparison with patients with the CG genotype. The statistical significance was set at P ≤ 0.05