Figure 1.

a) One‐photon fluorescence response with time for the reactions of SHP‐Cyto (1 μm) with H₂O₂ (1 mm). Spectra were acquired at 0 to 70 min after addition of H₂O₂. b) Fluorescence responses of 1 μm SHP‐Cyto to various reactive oxygen and nitrogen species (200 μm). Bars represent the integrated fluorescence ratios F _yellow/F _blue at 0 to 120 min after addition of each reactive species. Data were acquired at 25 °C in 30 mm MOPS, pH 7.4, with excitation wavelength 370 nm.