Fig. 5.

Cross-binding test and limit of detection quantification. (A) Fluorescence image series for an assay with only one type of conjugation antibodies preloaded. (B) Evaluation of cross-binding level in 12 human serum samples. No significant cross-binding between biomarkers was found. (C–E) Limit of detection for each marker in multiplex tests. Eight tests were performed at each data point and error bars show the SD at each data point. The assay has a limit of detection lower than the diagnostics cutoff for all three biomarkers.