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. 2017 Dec 5;114(51):13507–13512. doi: 10.1073/pnas.1714386114

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Fig. 1. — IRAK1 expression and purification. (A) Domain organizations of IRAK1 and IRAK4. DD, death domain; ProST, proline/serine/threonine-rich domain. (B) SDS-PAGE of the first-step Co-affinity purification. MW, molecular weight. (C) SDS-PAGE of the sample before and after His-tag removal by TEV protease treatment overnight at 4 °C. (D) Gel filtration profile of His-tag removed IRAK1 on a Superdex 75 10/300 column and the corresponding SDS-PAGE of the elution fractions. The degraded IRAK1 fragments coeluted with the nondegraded construct band.