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. 2017 Sep 28;14(6):6650–6658. doi: 10.3892/ol.2017.7099

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Copyright: © Tamura et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Association of SHISA2 with WNT5A in PC cells. (A) Western blot analysis demonstrated that expression levels of WNT5A were downregulated by SHISA2-knockdown and upregulated in SHISA2 overexpression. ACTB was used as a loading control (cell lysate). Silver stain was used as a loading control (medium). (B) Representative picture of PC (magnification, ×200). Immunohistological analysis revealed the co-expression of SHISA2 and WNT5A in serial sectionsof clinical PC tissues. ACTB was used as a loading control. SHISA2, shisa family member 2; PC, prostate cancer; WB, western blotting; WNT5A, protein Wnt-5a; ACTB, β-actin; si, short interfering.