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. 2017 Oct 10;14(6):7311–7317. doi: 10.3892/ol.2017.7157

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Expression of PDCD6 reversed the miR-124-induced cellular apoptosis. (A) The expression level of miR-124 was determined in SKOV3 and OCVAR3 cells following co-transfection of NC or miR-124 mimics and either the pcDNA3.1/PDCD6 or pcDNA3.1/vector. The expression level of miR-124 markedly increased in cells transfected with miR-124 mimics. Transfection with pcDNA3.1/PDCD6 restored the expression of PDCD6 in SKOV3 and OCVAR3 cells even with co-transfection of miR-124 mimics. (B) The expression levels of PDCD6 were determined in SKOV3 and OCVAR3 cells following co-transfection of NC or miR-124 mimics and either the pcDNA3.1/PDCD6 or pcDNA3.1/vector. The expression level of PDCD6 was markedly suppressed in cells transfected with miR-124 mimics. Transfection of pcDNA3.1/PDCD6 restored the PDCD6 expression level in SKOV3 and OCVAR3 cells even with co-transfection of miR-124 mimics. (C) The expression level of PDCD6 protein was determined in SKOV3 and OCVAR3 following co-transfection with NC or miR-124 mimics and either the pcDNA3.1/PDCD6 or pcDNA3.1/vector. The expression level of PDCD6 protein was suppressed in cells transfected with miR-124. Transfection with pcDNA3.1/PDCD6 restored the expression level of PDCD6 protein in SKOV3 and OCVAR3 cells even with co-transfection of miR-124 mimics. (D) Transfection of miR-124 mimics alone or with pcDNA3.1/vector induced apoptosis of SKOV3 and OCVAR3 cells. (E) The cell cycle status of SKOV3 and OCVAR3 cells following co-transfection of NC or miR-124 mimics and either the pcDNA3.1/PDCD6 or pcDNA3.1/vector was detected by flow cytometry analysis. NC, negative control; miR, microRNA; PDCD6, programmed cell death 6.