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. 2017 Sep 28;14(6):6477–6484. doi: 10.3892/ol.2017.7095

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Copyright: © Ma et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — HNF-4α is overexpressed and modulated in GC cell lines and their variants. (A) Nuclear proteins were exacted from 6 GC cell lines and HNF-4α expression levels of GC cells were compared with immortalized gastric epithelial cells using western blot analysis. (B) GC cell line SGC7901 and the multidrug-resistant strain SGC7901/VCR were stably infected with lentiviruses expressing HNF-4α, HNF-4α-specific siRNA or their corresponding controls. Western blotting was performed to determine the expression of HNF-4α in GC cell variants. β-actin served as a loading control. The results are representative of 4 independent experiments. HNF-4α, hepatocyte nuclear factor 4α; VCR, vincristine; Ctrl, control; GC, gastric cancer.