Figure 2.

HNF-4α has no clear influence on intracellular accumulation and retention of Adriamycin in gastric cancer cells. Gastric cancer cells and their variants were incubated with 5 mg/ml Adriamycin for 1 h. (A) Cells were directly harvested and subjected to flow cytometry to detect Adriamycin accumulation. (B) Cells were changed to drug-free medium for another 1 h and then harvested to detect Adriamycin retention. Intracellular Adriamycin was determined using flow cytometry and fluorescence intensity was expressed as the mean ± standard error of the mean. (C) The Adriamycin releasing index was calculated as: 100% × (mean fluorescence intensity of accumulation-mean fluorescence intensity of retention)/(mean fluorescence intensity of accumulation). Results were from 3 independent experiments. Ctrl, control; HNF4, hepatocyte nuclear factor 4; VCR, vincristine; siRNA, small interfering RNA.