Table I.

Screening for aberrantly activated TFs in chemo-resistant GC cells.

Transcription factor	Activity ratio (VCR/SGC7901)	P-value
NF-κB	2.17	0.006
HIF-1	1.95	0.026
HNF-4α	1.92	0.019
RXR	1.89	0.025
GATA	1.87	0.021
STAT1	1.74	0.031
STAT3	1.69	0.024
4-Oct	1.68	0.017
C/EBP	1.64	0.004
KLF4	1.58	0.011
Snail	1.56	0.028
NFAT	1.55	0.035
TCF/LEF	1.54	0.007
AP1	1.52	0.009
ATF2	1.5	0.016

Human GC cell line SGC7901 and its multidrug-resistant variant SGC7901/VCR were used to screen for TFs associated with chemo-resistance. The TF Activation Profiling Plate Array was employed to determine activities of 96 TFs in one plate. The activity of each TF was automatically recorded and 1.5 was set as the threshold value for screening over-activated TFs. GC, gastric cancer; TF, transcription factor; NF-κB, nuclear factor-κB; HIF-1, hypoxia-inducible factor 1; HNF-4α, hepatocyte nuclear factor4α; RXR, retinoid X receptor; GATA, GATA-binding protein; STAT, signal transducer and activator of transcription; 4-Oct, octamer-binding transcription factor 4; C/EBP, CCAAT/enhancer-binding protein; KLF4, Krüppel-like factor 4; NFAT, nuclear factor of activated T-cells; TCF/LEF, transcription factor/lymphoid enhancer-binding factor 1; AP1, activating protein-1; ATF2, activating transcription factor 2.