Table II.
Effects of HNF-4α on drug sensitivities of GC cells.
| IC50 values, µg/ml | |||||
|---|---|---|---|---|---|
| Cell line | ADR | 5-Fu | CDDP | VCR | MMC |
| SGC7901 | 0.52±0.05 | 3.11±0.22 | 1.63±0.21 | 0.25±0.01 | 1.39±0.16 |
| SGC7901-Ctrl | 0.61±0.11 | 2.89±0.34 | 1.93±0.28 | 0.29±0.08 | 1.56±0.23 |
| SGC7901-HNF4α | 1.58±0.29a | 6.98±0.73a | 4.16±0.52a | 1.73±0.31a | 4.55±0.67a |
| SGC7901/VCR | 5.44±0.47 | 9.17±1.15 | 7.65±0.94 | 5.86±0.69 | 8.22±1.03 |
| SGC7901/VCR-siCtrl | 6.21±0.79 | 10.83±1.57 | 7.02±0.86 | 6.29±0.88 | 9.03±1.27 |
| SGC7901/VCR-siHNF4α | 2.95±0.38b | 8.21±0.93b | 5.14±0.78b | 3.46±0.52b | 6.11±0.94b |
HNF4α was overexpressed in SGC7901 cells and suppressed in SGC7901/VCR cells with lentiviruses expressing HNF-4α or its specific small interfering RNA (siHNF4α). The sensitivity of SGC7901 and SGC7901/VCR cells, and their variants, was determined in vitro using an MTT assay. SGC7901-Ctrl and SGC7901/VCR-siCtrl cells were infected with control lentiviruses.
a
P<0.01 vs. SGC7901 and SGC7901-Ctrl.
b
P<0.01 vs. SGC7901/VCR and SGC7901/VCR-siCtrl. Results are representative of 3 independent experiments. IC50, half-maximal inhibitory concentration; Ctrl, control; ADR, Adriamycin; 5-Fu, 5-fluorouracil; CDDP, cisplatin; VCR, vincristine; MMC, mitomycin; HNF-4α, hepatocyte nuclear factor-4α.