Figure 4.

SOX4 is a direct target of miR-320. (A) Schematic representation of SOX4 3′-UTRs demonstrating the putative miR target site. The relative luciferase activity of (B) wt and (C) mu SOX4 3′-UTRs. The expression of SOX4 level in miR-320-overexpressing MDA-MB-231 and miR-320-depleted MCF7 cells, determined using (D) RT-qPCR and (E) western blot analysis. GAPDH was used as the loading control. ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. corresponding NC group. SOX4, SRY-box 4; miR, microRNA; UTR, untranslated region; NC, negative control; wt, wild-type; mu, mutated.