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. 2017 Sep 26;14(6):6869–6875. doi: 10.3892/ol.2017.7060

Figure 1.

Figure 1.

Establishment of a chidamide-resistant cell line. A549 cells were continuously exposed to gradually increasing chidamide concentrations of 4, 8, 16, 32 and 64 µM for ~6 months, and a chidamide-resistant lung cancer cell line (A549-CHI-R) was established. (A) Representative bright field images of parental A549 and A549-CHI-R cells. Cells were treated with or without 5 µM chidamide for 40 h. (B) Cell survival curves of A549 and A549-CHI-R cells. Cells were treated with a range of concentrations of chidamide for 72 h. (C) Colony formation of parental A549 and A549-CHI-R cells. Parental A549 cells exhibited decreased colony formation following 5 µM chidamide treatment for 10 days compared with A549-CHI-R cells. (D) Quantified colony formation assay for parental A549 and A549-CHI-R cells. ***P<0.001 between control and chidamide-treated cells. A549-CHI-R, chidamide-resistant A549 cells; DMSO, dimethyl sulfoxide; CHI, chidamide; ns, no significance.