Figure 2. Upregulation of multiple signaling pathways in ZMYM2-FGFR1 induced AML.
a) Quantitative RT-PCR analysis shows the comparison of gene expression levels in ZMYM2-FGFR1 mice (ZMYM2-NSG) compared with normal, healthy human PB mononuclear cells (Normal PBMC). b) Western blot analysis shows protein levels of MYC, BCL2, and an FGFR1 downstream signaling intermediate, phospho-STAT5, in leukemic mouse spleens compared with normal human PBMC and normal NSG mouse spleens. c) RT-PCR analysis shows the transcriptional levels of genes in AML cells from a ZMYM2-FGFR1 patient-derived AML compared with PBMC from a healthy donor.