Figure 1.

LncTCF7 level is highly expressed in human CRC tissues. 58 CRC patient surgery samples were studied. Paired cancer tissues and adjacent normal mucosal tissues from each patient were subjected to the following experiments: (A) Total RNA from each tissue was harvested to detect lncTCF7 level by qRT-PCR. 18S RNA served as internal control. Error bar, mean ± SEM (****P<0.0001). compared to peri-tumor tissue. (B) lncTCF7 level and localization was studied by RNA FISH assay on tumor and peri-tumor tissue. Frozen sectioned tissues on slides were permeabilized and hybridized with FITC-labeled LncTCF7 probe. Nuclei were stained with DAPI. Green, lncTCF7; Blue nuclei. Scale bar, 25 µm. (C) TCF7 protein levels in tumor and peri-tumor samples were represented by western blot analysis on representative case. LncTCF7, Long non-coding RNA TCF7, CRC, colorectal cancer.