Fig. 2.

Analysis of the longitudinal 454 deep sequencing data. a Analysis of the frequency of all non-synonymous mutations detected by deep sequencing at the 18 codons associated with raltegravir resistance. Only unique variants with a minimum of 10 reads were included in the analysis. Total number of reads and the proportion of reads containing the denoted mutations relative to the total number of reads are given. Mutations of interest are highlighted by colored boxes. Similar colored boxes are mutations that appeared to be on the same genome. Red boxes indicate mutations from the Q148 pathway, yellow boxes indicate the Y143 pathway and green boxes the N155 pathway. b Sequences containing multiple mutations are shown. Double mutants are sorted by frequency