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Metformin and DDP induce apoptosis in ovarian cancer cells. Cell apoptosis was measured by flow cytometry analysis. HO-8910 cells were treated with (A) metformin (0.01, 0.5, 1, 5, or 10 mM) or (B) DDP (5 µM) or a combination of the two (0.5, 1, or 5 µM metformin plus 5 µM DDP) for 48 h to examine their effects on apoptosis. ***P<0.001 compared with control. ##P<0.01 and ###P<0.001 vs. DDP. DDP, cisplatin.
