Fig. 3.

Luciferase reporter assays demonstrating the target relationship between miR-494 and APC mRNA. a Predicted miRNA binding sites within the 3′-UTR of APC mRNA. The pairing between miR-494 and the putative binding sites in the 3′-UTR of APC mRNA are shown. Mutations in the APC 3′-UTR are underlined. b Luciferase activity, normalized to renilla luciferase activity, was measured in homogenates of HEK293T cells transfected with the wild-type 3′-UTR and mutant 3′-UTR APC luciferase constructs and a miR-494 mimic or a scrambled miRNA control. c APC mRNA expression is reduced by the miR-494 mimic and increased by the miR-494 inhibitor. d Expression of APC protein is reduced by the miR-494 mimic and increased by the miR-494 inhibitor. (*, P < 0.05, Student’s t-test, n = 3 independent experiments)