Fig. 5.

miR-494 induces β-catenin signaling (Top/Fop, c-Myc, cyclin D1). a Dual luciferase assay demonstrating the effect on Top/Fop reporter activity in HCT-116 cells. Values were normalized to a renilla transfection control. The figure shown is a representative of at least three independent experiments. b Dual luciferase assay showing the effect of expression of a miR-494 mimic on a c-Myc reporter construct in HCT-116 cells. c Dual luciferase assay showing the effect of expression of a miR-494 mimic on a cyclin D1 reporter construct in HCT-116 cells. d Western blot analysis of β-catenin expression in the cytoplasm (C) and nucleus (N) of the indicated cells. α-tubulin and fibrillarin were measured by western blot to monitor the efficiency in the preparation of cytosolic and nuclear protein extracts, respectively. e Western blot analysis of β-catenin and cyclin D1 in the indicated cells. (*, P < 0.05, Student’s t-test, n = 3 independent experiments)