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Fluorescence visualization of ADM intracellular accumulation. (A) HU7 and HT22 cells were treated with free RBITC solution or RBITC-LMCC nanoparticles. Fluorescent microimages of the cells were obtained at 1, 2 and 4 h. (B) HU7 cells were treated with various concentrations (150, 300 and 600 µg/ml) of RBITC-LMCC nanoparticles for 1, 2, and 4 h, respectively. Scale bars, 10 µm. ADM, Adriamycin; RBITC, rhodamine B isothiocyanate.
