Figure 1.
Irradiation induces the expression of MUC1 in hepatocellular carcinoma cells. (A) Western blot analysis of MUC1 protein in SMMC-7721 cells 24 h after exposure to indicated doses of irradiation. (B) Reverse transcription-quantitative polymerase chain reaction analysis of MUC1 mRNA abundance in SMMC-7721 cells exposed to 8 Gy irradiation and incubated for different times. (C) Western blot analysis of indicated proteins in SMMC-7721 cells exposed to 8 Gy irradiation for different times. Bar graphs present data from three independent experiments performed in triplicates. Data are expressed as the mean ± standard deviation. *P<0.05 vs. non-irradiated control. MUC1, Mucin 1; STAT3, signal transducer and activator of transcription 3; P, phosphorylated.