Figure 3.

MUC1 overexpression suppresses irradiation-induced apoptosis. (A) SMMC-7721 cells were exposed to 8 Gy irradiation. Following incubation for 48 h, cells were stained with Annexin V and PI. The percentage of Annexin-V-positive apoptotic cells was determined. (B) Western blot analysis of the levels of cleaved caspase-3 and cleaved PARP in SMMC-7721 cells. Values are results from representative experiments performed in triplicates. Data are expressed as the mean ± standard deviation. *P<0.05 vs. non-irradiated counterparts; ^#P<0.05 vs. vector-transfected cells with irradiation. MUC1, Mucin 1; PARP, poly (ADP-ribose) polymerase; PI, propidium iodide.