Fig. 6.

ROS accumulation and SOD, CAT and POD enzymatic activities in developing anthers. The superoxide anion, hydrogen peroxide and MDA content in MF-1376 and PHYMS anthers was measured at the tetrad, mononuclear and trinuclear stage of development. The enzymatic actives of superoxide dismutase, peroxidase and catalase in MF-1376 and PHYMS anthers were also measured at each stage. A single sample was used for three independent replicates (n = 3). *, ** = significantly different from the MF-1376 control at p < 0.05 and p < 0.01, respectively