Fig. 3.

SAHA restores Pa-LPS mediated decrease in FoxP3+ T-reg cells. The Cftr^+/+ mice were i.t. instilled with Pa-LPS (20 μg/mouse) for total of 36 h (n = 3). Mice were i.t. treated with SAHA (50 μg/mouse in 100 μl total volume of PBS), for 12 h post Pa-LPS challenge. a The BALF cells were harvested and analyzed for CD4+ (CD4-PE antibody) and FoxP3+ [(FoxP3 (Rabbit polyclonal primary Ab)-Anti-Rabbit-FITC (secondary Ab)] cells by flow cytometry using the BD FACS Caliber instrument. Data indicates that SAHA treatment significantly (p < 0.05) induces the levels of FoxP3+ T regs to counteract the Pa-LPS mediated lung inflammation. The data represents mean ± SD from three independent experiments. b Immunostaining of longitudinal lung sections from the same groups of Cftr^+/+ mice show that SAHA induces FoxP3 expression (white arrows) that implicates elevated T-reg numbers in the lungs to protect against Pa-LPS induced inflammatory disease