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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Curr Opin Chem Biol. 2014 Jan 29;18:55–61. doi: 10.1016/j.cbpa.2013.12.017

Figure 1.

Figure 1

Utilization of defined glycan microarrays to elucidate GBP specificity. Libraries of well-characterized glycans generated by release of defined glycans from glycoproteins, other natural sources or by chemical or chemoenzymatic synthesis are used to populate well-defined glycan microarrays. Structures reflect naturally occurring glycans and modifications of glycans not typically found in nature. Glycan libraries undergo derivatization with a functional coupling moiety, followed by printing in a microarray format to generate the glycan microarray. GBPs are incubated with the glycan microarrays over different concentrations and detected by fluorescence emission if directly labeled or by a similarly labeled suitable secondary detecting agent. While many approaches can be taken to analyze glycan array data, examination of GBP binding over a variety of concentrations for individual glycans is shown.