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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Curr Opin Chem Biol. 2014 Jan 29;18:55–61. doi: 10.1016/j.cbpa.2013.12.017

Figure 2.

Figure 2

Using shotgun glycan microarrays to identify native glycan ligands for GBPs. Glycans released from natural sources, such as red blood cells or neutrophils shown in the schematic, are derivatized with a functional linker, subjected to multidimensional chromatography and printed in a microarray format. Following interrogation of the library with GBP, glycan fractions isolated following multidimensional chromatography that correspond to GBP bound ligands are subjected to mass spec glycan sequencing to identify native glycans recognized by GBP.