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Using microbial glycans to examine host pathogen interactions. Glycans, in particular lipopolysaccharide O antigens, released from well-characterized microbes, undergo derivatization and printing in a microarray format. GBPs with putative pathogen recognition activity or sera are incubated with the microarray to examine potential interactions with microbial glycans. Positive interactions are confirmed by examining GBP or sera with intact microbe.
