Figure 2. Influence of the LAT1-specific inhibitor JPH-203 on RBC-EC adhesion as a function of shear stress.
The adhesion to HUVECs of fresh human RBCs exposed or not to JPH-203 (50 nM) was quantified in customized flow chambers at varying shear stress. JPH-203 (50 nM) was also included in the perfusion medium. One set of individual adhesion curves is shown in (A) and the mean results from n=5 experiments each at 1, 2, or 5 dynes/cm2 in (B). *, p<0.05 vs. Con (vehicle control) by paired t-test; comparison of Con vs. JPH-203 curves is by two-way ANOVA.