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. Author manuscript; available in PMC: 2018 Jan 5.
Published in final edited form as: Thromb Haemost. 2017 Apr 6;117(7):1402–1411. doi: 10.1160/TH16-05-0373

Figure 3. LAT1 is present in fresh and stored human RBCs.

Figure 3

(A), Immunoblots detecting LAT1 (Santa Cruz Ab sc-374232) in human RBC membranes stored either 0 days or 38 days. Lower panel shows Coomassie protein staining after gel was stripped (loading control). Results are typical of n=4 experiments. (B), mRNA in reticulocytes. Magnetic beads and CD71 (transferrin) antibodies were used to isolate reticulocytes and RT-PCR was performed for LAT1 message. LAT1 mRNA is detected in reticulocytes. Left lane, PCR ladder; other lanes are sample replicates.