Fig. 5.
Effect of p57KIP2 gene knockout on induction of apoptosis by combination treatment. a Protein expression of p57 and PARP1 in parental and knockout VM-Cub1 cells with or without combination treatment. b Relative viability of VM-Cub1 parental and p57 knockout cells after combination treatment. Differences between samples with combination treatment of parental and knockout cells were analyzed using Student’s t test (***p ≤ 0.001). c Examples of clonogenicity assay, with quantified colony counts (***p ≤ 0.001) and d cell cycle analysis for the indicated cells following combined inhibitor treatment. See Additional file 3 for cell numbers in the respective cell cycle phases