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Incubation of leukocytes with galectins induces agglutination. Promyelocytic HL60 cells were incubated with PBS or 10 μM galectin-1 (Gal-1) for 4 h followed by microscopic evaluation for agglutination (no lactose). Following incubation with PBS or Gal-1, lactose was added at a final concentration of 20 mM lactose for 15 min at 37 °C followed by microscopic evaluation as indicated
