Skip to main content
. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Cancer Res. 2017 Sep 18;15(12):1777–1791. doi: 10.1158/1541-7786.MCR-17-0004

Figure 4. YAP suppression in vivo decreases Notch signaling and SOX2 levels.

Figure 4

(A) YAP shRNAs decrease HES1 expression in SMS-CTR xenograft tumors as assessed by qRT-PCR. (B) Representative images of SOX2 IHC in the tumors. YAP, Ki67, and SOX2 IHC were scored on a scale of 0–3 for all tumors (NT, YAP_sh3, YAP_sh4). The r value represents a positive and significant correlation of expression of (C) YAP and Ki67, (D) YAP and SOX2, and (E) SOX2 and Ki67. (F) Limiting dilution assay in RD spheres after YAP suppression. The average sphere-forming frequency is shown, with the expected range in parentheses. NT= non-targeting control. ***, P<0.001; and ****, P<0.0001.