(A, B, top) Expression of the dox-inducible YAP5SA construct increases total YAP protein levels in RD and SMS-CTR spheres as assessed by immunoblot. (A, B, bottom) Spheres quantified. (C, D, left) Increasing doses (0µM, 3µM, 30µm) of GSI RO4929097 inhibits sphere formation in RD and SMS-CTR spheres. (C, D right) Expression of YAP5SA in the presence of GSI restores sphere formation. (E, F)
HES1 and HEY1 levels are decreased after GSI treatment in RD and SMS-CTR spheres, shown by qRT-PCR. *, P<0.05; **, P<0.01; ***, P<0.001; and ****, P<0.0001. Scale bars: 1.7mm.