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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Mol Cancer Res. 2017 Sep 18;15(12):1777–1791. doi: 10.1158/1541-7786.MCR-17-0004

Figure 6. YAP expression rescues pharmacologic Notch inhibition.

Figure 6

(A, B, top) Expression of the dox-inducible YAP5SA construct increases total YAP protein levels in RD and SMS-CTR spheres as assessed by immunoblot. (A, B, bottom) Spheres quantified. (C, D, left) Increasing doses (0µM, 3µM, 30µm) of GSI RO4929097 inhibits sphere formation in RD and SMS-CTR spheres. (C, D right) Expression of YAP5SA in the presence of GSI restores sphere formation. (E, F) HES1 and HEY1 levels are decreased after GSI treatment in RD and SMS-CTR spheres, shown by qRT-PCR. *, P<0.05; **, P<0.01; ***, P<0.001; and ****, P<0.0001. Scale bars: 1.7mm.