| LAMP components (initial concentration) | Volume (μL) |
| ddH2O | 11.6 |
| MgSO4 (100 mM) | 2.0 |
| dNTPs (25 mM) | 1.4 |
| Betaine (5 M) | 4.0 |
| Buffer (10x) | 2.5 |
| Calcein (1.25 mM) | 0.5 |
| MnCl2 (25 mM) | 0.5 |
| Bst polymerase (8 U/μL) | 1.5 |
| plant DNA (10 ng/μL) | 1.0 |
| Total volume | 25.0 |
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