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. 2018 Jan 4;19:16. doi: 10.1186/s12864-017-4408-9

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Total genomic and plasmid extractions of strains were subjected to electrophoresis (a) and probed with pthA probe (using primer pthAF/R DIG as the probe), Xc-A306-p33 and Xc-A306-p64 plasmid specific probes by Southern hybridization (b, c and d, respectively). Lanes 1 and 17 = DNA Molecular Weight Marker III DIG labeled; 2 = Erwinia stewartii SW2; 3 = Xc-A306 plasmid extraction (pXc-A306); 4 = Xc-A306 genomic DNA restricted with EcoRI (Xc-A306 EcoRI); 5 = pXc-03-1638-1-1; 6 = Xc-03-1638-1-1 EcoRI); 7 = pXc-A2090; 8 = Xc-A2090 EcoRI; 9 = pXc-A1660; 10 = Xc-A2090 EcoRI; 11 = pXc-AEtrog; 12 = Xc-AEtrog EcoRI; 13 = pXc-A100-Japan; 14 = Xc-A100 Japan EcoRI; 15 = pXc-A109 India; 16 = Xc-A109 India EcoRI. A = Ethidium bromide stained gel (agarose 0.7%). b Southern blot of gel in A with pthA probe. c Southern blot of gel in A with Xc-p33 probe (using primer SPCF33F/R DIG as the probe). d Southern blot of gel in A with Xc-p64 probe (using primer SPCF64F/R DIG as the probe)