| RBC species | Chicken | Turkey | Guinea pig | Human type O |
| Concentration of RBCs (v/v) | 0.75% | 0.75% | 1% | 1% |
| Type of microtiter plate | V bottom | V bottom | U bottom | U bottom |
| Incubation time, RT | 30 min | 30 min | 1 hour | 1 hour |
| Appearance of non-agglutinated cells | Button* | Button* | halo | halo |
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