Marker	Clone	Company	Dilution	Dewaxing	Antigen retrieval	Antibody incubation	Scoring system
ER	SP1	Ventana	RTU	EZ prep at 72 °C	cc1 at 95 °C for 36 min	16 min	ASCO/CAP guidelines23
PR	1E2	Ventana	RTU	EZ prep at 72 °C	cc1 at 95 °C for 36 min	17 min	ASCO/CAP guidelines23
HER2	4B5	Ventana	RTU	EZ prep at 72 °C	cc1 at 95 °C for 36 min	18 min	ASCO/CAP guidelines3
Ki67	30-9	Ventana	RTU	EZ prep at 72 °C	cc1 at 95 °C for 36 min	12 min	International Ki67 in Breast Cancer working group recommendations25