| Reganet | Volume (µL) |
| Ultra Pure Water | 478 |
| 1 M Tris-HCl pH 7.5 | 10 |
| 0.5 M EDTA | 10 |
| 5 M NaCl | 2 |
| Glycerol | 500 |
| Total Volume | 1,000 |
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