Reagent	Volume (µL)
Ultra Pure Water	3.3
10x Restriction Endonuclease Buffer (e.g. NEBuffer)	5
25 mM ATP	2
10 mM dNTPs	2
T4 Polynucleotide Kinase (10 U/µl)	1
T4 DNA polymerase (3 U/µl)	1.5
DNA polymerase I, Large (Klenow) Fragment (5 U/µl)	0.2
Total Volume	15