Buffer Composition

A.1.  Immunoprecipitation buffer (IP)

20 mM Tris-HCl pH 7.5

2 mM EDTA

150 mM NaCl

0.1% Triton X-100

0.1% Deoxycholate

10  mM Sodium Butyrate

A.2. Low Salt Wash buffer

20 mM Tris-HCl pH 8.0

2 mM EDTA

150 mM NaCl

1% Triton X-100

0.1% SDS

A.3. High Salt Wash buffer

20 mM Tris-HCl pH 8.0

2 mM EDTA

500 mM NaCl

1% Triton X-100

0.1% SDS

A.4. ChIP Elution buffer

100 mM NaHCO3

1% SDS

A.5. 1x Lysis buffer – 1 mL

0.1% Triton

0.1% Deoxycholate

10 mM Sodium Butyrate

A.6.  Ab dilution buffer

0.05% (w/v) Azide

0.05% broad spectrum antimicrobial (e.g. ProClin 300)

in PBS

A.7. 30% PEG/1M NaCl Magnetic Bead Solution (reference16)

30% PEG

1 M NaCl

10 mM Tris HCl pH 7.5

1 mM EDTA

1 mL of washed super-paramagnetic beads

A.8. 20% PEG/1M NaCl Magnetic Bead Solution (reference16)

30% PEG

1 M NaCl

10 mM Tris HCl pH 7.5

1 mM EDTA

1 mL of washed  super-paramagnetic beads