Skip to main content
. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Nov 24;1860(2):586–599. doi: 10.1016/j.bbamem.2017.11.014

Fig. 3. CTII but not DCCD-BPF can bind to liposomes enriched in CL.

Fig. 3

1H-NMR spectra derived from the N+(CH3)3 groups of PC in sonicated unilamellar liposomes composed of PC, PA, PS and CL in molar ratio 6.5, 0.6, 0.4 and 2.5 respectively at a total lipid concentration 1.4 × 10−2 M in the presence of K3Fe(CN)6 (10 μl of saturated K3Fe(CN)6 solution per 1 ml of liposomes) at 15 °C. Control 1H-NMR spectra (a and d) derived from liposomes incubated for 30 min in a tube for 1H-NMR prior to adding K3Fe(CN)3. The other 1H-NMR spectra were recorded from liposomes incubated with 1×10−4 M CTII (b) or 2 × 10−4 M CTII (f) or 2 ×10−4 M DCCD-binding protein (e) for 30 min prior to addition of K3Fe(CN)6. 1H-NMR spectra (c and g) derived from liposome samples used in panels b and f respectively but recorded 1 hour later. Each 1H-NMR spectrum shows representative 1H-NMR traces from three independent experiments that showed similar results. Each sample (a – g) was measured in triplicate.