Step | Temp | Time | Solution |
1 | -140 °C to -9 0°C | 30 min | Liquid nitrogen |
2 | -90 °C | 96 hr | FS cocktail |
3 | -90 °C to -60 °C | 6 hr (5 °C/hr) | FS cocktail |
4 | -60 °C | 12 hr | FS cocktail |
5 | -60 °C to -25 °C | 7 hr (5 °C/hr) | FS cocktail |
6 | -25 °C | 12 hr | FS cocktail |
7 | -25 °C to 0 °C | 5 hr (5 °C/hr) | FS cocktail |
8 | 0 °C | 1 hr x 3 times | Acetone |
9 | Room temp | Resin infiltration |