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. 2017 Dec 19;(130):56567. doi: 10.3791/56567
Step Temp Time Solution
1 -140 °C to -9 0°C 30 min Liquid nitrogen
2 -90 °C 96 hr FS cocktail
3 -90 °C to -60 °C 6 hr (5 °C/hr) FS cocktail
4 -60 °C 12 hr FS cocktail
5 -60 °C to -25 °C 7 hr (5 °C/hr) FS cocktail
6 -25 °C 12 hr FS cocktail
7 -25 °C to 0 °C 5 hr (5 °C/hr) FS cocktail
8 0 °C 1 hr x 3 times Acetone
9 Room temp Resin infiltration