Affinity chromotography parameter
Measuring parameters	UV (280 nm), Elution buffer concentration, Conductance
Settings	Variable/Unit	Value
Flow rate	mL/min	1
Maximum column pressure	MPa	0.35
Wavelength	nm	280 nm
Binding buffer	Pump position	A
Elution buffer	Pump position	B
Starting concentration elution buffer	%	0
System volume compensation	mL	10
Column equilibration	mL	20
Sample injection over superloop/syringe	mL	cell lysate volume
Washing step	mL	20-35
Elution step	mL	20-35
Concentration elution buffer during elution	%	100
Recalibration of column with 20% EtOH	mL	50
Desalting parameter
Measuring parameters	UV (280 nm), Conductance
Settings	Variable/Unit	Value
Flow rate	mL/min	4
Maximum column pressure	MPa	0.35
Wavelength	nm	280 nm
Binding buffer	Pump position	A
Elution buffer	Pump position	B
Starting concentration elution buffer	%	0
System volume compensation	mL	10
Column equilibration	mL	20
Sample injection over superloop/syringe	mL	cell lysate volume
Desalting buffer injection (0.8 M sorbitol)	mL	100
Recalibration of column with 20% EtOH	mL	50