Figure 2. E1A 1-80 activates three panels of genes by targeting the MYC-NuA4 complex and p300 separately and cooperatively.

A. Activation of three panels of genes within those activated by E1A 1-80FH identified by RNA-seq analysis. The MNA4 panel genes are defined by three criteria: i) activation by E1A 1-80FH of ≥ 35%, and ii) a ratio of activation by ∆2-11 /activation by E1A 1-80FH of ≥ 65%, and iii) a ratio of activation by ∆26-35/activation by ∆2-11 of ≤ 40%. The p300 panel genes are defined similarly and overlap between activation by ∆26-35 and activation by E1A 1-80FH. The MNP300 panel genes are defined by activation of ≥ 35% by E1A 1-80FH, and an activation ratio of ≤ 40% for both ∆2-11 (∆2-11 activation/E1A 1-80FH activation) and for ∆26-35 (∆26-35 activation/ E1A 1-80FH activation). PANTHER (version 12) gene enrichment analysis [32–34] was performed online (http://www.geneontology.org). B. Activation of selected MNA4 panel genes involved in ribosome biogenesis/gene expression by RT-qPCR analysis. The three sets of RNA used for RNA-seq analysis were used for RT-qPCR analysis with gene specific primers. Graph represents average RT-qPCR results from the three sets of RNA samples with standard deviations shown. C. Activation of selected MNP300 panel genes involved in DNA replication/cell cycle by E1A 1-80FH. These genes were activated by ∆2-11 and ∆26-35 only poorly. Conditions were the same as in B. D. Average activation of genes involved in ribosome biogenesis/gene expression from the MNA4 panel (157 genes total). Data were derived from RNA-seq results. E. Average activation of genes involved in DNA replication/cell cycle from the MNP300 panel (82 genes total).