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. 2018 Jan 5;13(1):e0189188. doi: 10.1371/journal.pone.0189188

Fig 3. Bray Curtis dissimilarity between PCR replicates and between high and low molecular weight DNA fractions in the degradation experiment and from Malaise trap samples.

Fig 3

A) Bray Curtis dissimilarity between PCR replicates of the same mock community samples at 5°C different annealing temperatures (51°C from 46°C), with a reduced PCR cycle number (22 x from 32 x), using a different forward primer (mlCOIintF instead of ARF1), and for two exact replicates under identical conditions (to test for stochastic PCR bias). B) Bray Curtis dissimilarity between high and low molecular weight DNA fractions for community samples stored under freezer (FT) and room temperature (RT) conditions or from an actual Malaise trap (M) and amplified for mitochondrial COI and C) for microbial 16SrDNA.