Table 1. Summary of PCR condition experiment.

The table shows sample size, primer combinations, annealing temperature and PCR cycle number as well as average alpha–and beta diversity ± standard deviation. The first two rows are repeated and present an exact replicate at the same PCR conditions. Significant differences are indicated by shading of cells.

N	Primer combination	Tanneal	# cycles	SR	Alpha div.	Beta div.
15	ARF1/Fol-degen-rev	46°C	32	39.07 ± 15.44	0.88 ± 0.05	0.05 ± 0.01
15	ARF1/Fol-degen-rev	46°C	32	40.07 ± 14.70	0.88 ± 0.04
15	mlCOIintF/Fol-degen-rev	46°C	32	42.40 ± 15.02	0.90 ± 0.04	0.33 ± 0.08
15	ARF1/Fol-degen-rev	51°C	32	39.00 ± 14.90	0.86 ± 0.05	0.61 ± 0.14
15	ARF1/Fol-degen-rev	46°C	22	41.82 ± 16.26	0.92 ± 0.03	0.64 ± 0.09